In contrast to ubiquitin, SUMO is not used to tag proteins for degradation. Mature SUMO is produced when the last four amino acids of the C-terminus have ...
Our SUMO-tag expression system maximizes the yield of soluble, functional proteins in E. coli. SUMO functions as both a chaperonin and initiator of protein ...
由 D Kuo 著作 · 2014 · 被引用 45 次 — SUMO as a solubility tag and in vivo cleavage of SUMO fusion proteins with Ulp1. Methods Mol Biol. 2014:1177:71-80. doi: 10.1007/978-1-4939-1034-2_6.
Specifications · Species of Origin. Human · Affinity Tag. His-SUMO Tag (N-term) · Purity. >98% as determined by SDS-PAGE analysis. · Endotoxin level.
由 TR Butt 著作 · 2005 · 被引用 653 次 — However, in eukaryotic systems, endogenous SUMO proteases immediately cleave SUMO fusions, making purification using the SUMO tag impossible. A novel fusion ...